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isotype control antibody  (R&D Systems)


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    Structured Review

    R&D Systems isotype control antibody
    Isotype Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+igg2a+isotype+control+antibody/Mouse+IgG2A+Isotype+Control/pm42009143-90-26-31
    Average 93 stars, based on 89 article reviews
    isotype control antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Siglec-9 is an inhibitory receptor on human mast cells in vitro.
    Article Snippet: Background: Mast cell activation is critical for the development of allergic diseases.. Ligation of sialic acid–binding immunoglobin-like lectins (Siglecs), such as Siglec-6, -7, and -8 as well as CD33, have been shown to inhibit mast cell activation.. Recent studies showed that human mast cells express Siglec-9, an inhibitory receptor also expressed by neutrophils, monocytes, macrophages, and dendritic cells.

    Binding Assay:

    Article Title: Siglec-9 is an inhibitory receptor on human mast cells in vitro.
    Article Snippet: Background: Mast cell activation is critical for the development of allergic diseases.. Ligation of sialic acid–binding immunoglobin-like lectins (Siglecs), such as Siglec-6, -7, and -8 as well as CD33, have been shown to inhibit mast cell activation.. Recent studies showed that human mast cells express Siglec-9, an inhibitory receptor also expressed by neutrophils, monocytes, macrophages, and dendritic cells.

    Incubation:

    Article Title: Siglec-9 is an inhibitory receptor on human mast cells in vitro.
    Article Snippet: Background: Mast cell activation is critical for the development of allergic diseases.. Ligation of sialic acid–binding immunoglobin-like lectins (Siglecs), such as Siglec-6, -7, and -8 as well as CD33, have been shown to inhibit mast cell activation.. Recent studies showed that human mast cells express Siglec-9, an inhibitory receptor also expressed by neutrophils, monocytes, macrophages, and dendritic cells.

    Control:

    Article Title: Siglec-9 is an inhibitory receptor on human mast cells in vitro.
    Article Snippet: Background: Mast cell activation is critical for the development of allergic diseases.. Ligation of sialic acid–binding immunoglobin-like lectins (Siglecs), such as Siglec-6, -7, and -8 as well as CD33, have been shown to inhibit mast cell activation.. Recent studies showed that human mast cells express Siglec-9, an inhibitory receptor also expressed by neutrophils, monocytes, macrophages, and dendritic cells.

    Article Title: A pan-orthohantavirus preclinical human lung xenograft mouse model
    Article Snippet: Endothelial 518 cells were stained with a mouse monoclonal anti-Von Willebrand factor antibody (1:50, clone 519 F8/86, Invitrogen, Waltham, United States), epithelial cells with a mouse monoclonal anti-520 cytokeratin 19 antibody (1:250, clone A53-B/A2, Abcam, Cambridge, United Kingdom), 521 immune cells with a mouse monoclonal anti-CD45 antibody (1:50, clone 2B11+PD7/26, 522 Agilent, Santa Clara, United States), protocadherin-1 with a mouse monoclonal anti-523 protocadherin-1 antibody (1:25, clone B-11, Santa Cruz Biotechnology, Dallas, United 524 States), and CD55 with a mouse monoclonal anti-CD55 antibody (1:25, clone NaM16-4D3, 525 Santa Cruz Biotechnology, Dallas, United States). .. As negative controls, corresponding 526 isotype control antibodies in corresponding dilutions were utilized with mouse IgG1 isotype 527 control antibody (RnD Systems, Minneapolis, United States) for endothelial cells, immune 528 cells, protocadherin-1 and CD55 and mouse IgG2a isotype control antibody (RnD Systems, 529 Minneapolis, United States) for epithelial cells. .. For β3 integrin staining, samples were 530 20 blocked with 10% normal goat serum (DAKO, Santa Clara, United States), primary antibody 531 was a rabbit monoclonal anti-β3 integrin antibody (1:50, clone SJ19-09, Novus Biologicals, 532 Abingdon, United Kingdom) followed by secondary HRP labeled goat anti-rabbit IgG 533 antibody (1:100, DAKO, Santa Clara, United States).

    Article Title: Human Leukocyte Antigen-Presented Macrophage Migration Inhibitory Factor is a Surface Biomarker and Potential Therapeutic Target for Ovarian Cancer
    Article Snippet: BB7.2 and RL21A hybridomas were grown in Hybridoma-SFM (Invitrogen), and antibodies were purified using protein G (GE Healthcare, Piscataway, NJ). .. Mouse IgG2a isotype control antibody was purchased from R&D Systems (20102; Minneapolis, MN). .. Mouse IgG2b isotype control antibody was purchased from BioLegend (MG2b-57; San Diego, CA).

    Article Title: Development of novel highly sensitive methods to detect endogenous cGAMP in cells and tissue.
    Article Snippet: Intracellular DNA triggers interferon release during the innate immune response.. Cyclic GMP-AMP synthase (cGAS) senses intracellular double-stranded DNA not only in response to viral infection but also under autoimmune conditions.. Measuring the levels of cyclic GMP-AMP (cGAMP) as a second messenger of cGAS activation is important to elucidate the physiological and pathological roles of cGAS.

    Article Title: A pan-orthohantavirus human lung xenograft mouse model and its utility for preclinical studies
    Article Snippet: Endothelial cells were stained with a mouse monoclonal anti-Von Willebrand factor antibody (1:50, clone F8/86, Invitrogen, Waltham, United States), epithelial cells with a mouse monoclonal anti-cytokeratin 19 antibody (1:250, clone A53-B/A2, Abcam, Cambridge, United Kingdom), immune cells with a mouse monoclonal anti-CD45 antibody (1:50, clone 2B11+PD7/26, Agilent, Santa Clara, United States), protocadherin-1 with a mouse monoclonal anti-protocadherin-1 antibody (1:25, clone B-11, Santa Cruz Biotechnology, Dallas, United States), and CD55 with a mouse monoclonal anti-CD55 antibody (1:25, clone NaM16-4D3, Santa Cruz Biotechnology, Dallas, United States). .. As negative controls, corresponding isotype control antibodies in corresponding dilutions were utilized with mouse IgG1 isotype control antibody (RnD Systems, Minneapolis, United States) for endothelial cells, immune cells, protocadherin-1 and CD55, and mouse IgG2a isotype control antibody (RnD Systems, Minneapolis, United States) for epithelial cells. .. For β3 integrin staining, samples were blocked with 10% normal goat serum (DAKO, Santa Clara, United States), primary antibody was a rabbit monoclonal anti-β3 integrin antibody (1:50, clone SJ19-09, Novus Biologicals, Abingdon, United Kingdom) followed by secondary HRP labeled goat anti-rabbit IgG antibody (1:100, DAKO, Santa Clara, United States).

    Article Title: A pan-orthohantavirus human lung xenograft mouse model and its utility for preclinical studies.
    Article Snippet: Endothelial cells were stained with a mouse monoclonal anti-Von Willebrand factor antibody (1:50, clone F8/86, Invitrogen, Waltham, United States), epithelial cells with a mouse monoclonal anti-cytokeratin 19 antibody (1:250, clone A53-B/A2, Abcam, Cambridge, United Kingdom), immune cells with a mouse monoclonal anti-CD45 antibody (1:50, clone 2B11+PD7/26, Agilent, Santa Clara, United States), protocadherin-1 with a mouse monoclonal anti-protocadherin-1 antibody (1:25, clone B-11, Santa Cruz Biotechnology, Dallas, United States), and CD55 with a mouse monoclonal anti-CD55 antibody (1:25, clone NaM16-4D3, Santa Cruz Biotechnology, Dallas, United States). .. As negative controls, corresponding isotype control antibodies in corresponding dilutions were utilized with mouse IgG1 isotype control antibody (RnD Systems, Minneapolis, United States) for endothelial cells, immune cells, protocadherin-1 and CD55, and mouse IgG2a isotype control antibody (RnD Systems, Minneapolis, United States) for epithelial cells. .. For β3 integrin staining, samples were blocked with 10% normal goat serum (DAKO, Santa Clara, United States), primary antibody was a rabbit monoclonal anti-β3 integrin antibody (1:50, clone SJ19-09, Novus Biologicals, Abingdon, United Kingdom) followed by secondary HRP labeled goat anti-rabbit IgG antibody (1:100, DAKO, Santa Clara, United States).

    Staining:

    Article Title: Siglec-9 is an inhibitory receptor on human mast cells in vitro.
    Article Snippet: Background: Mast cell activation is critical for the development of allergic diseases.. Ligation of sialic acid–binding immunoglobin-like lectins (Siglecs), such as Siglec-6, -7, and -8 as well as CD33, have been shown to inhibit mast cell activation.. Recent studies showed that human mast cells express Siglec-9, an inhibitory receptor also expressed by neutrophils, monocytes, macrophages, and dendritic cells.

    Concentration Assay:

    Article Title: Development of novel highly sensitive methods to detect endogenous cGAMP in cells and tissue.
    Article Snippet: Intracellular DNA triggers interferon release during the innate immune response.. Cyclic GMP-AMP synthase (cGAS) senses intracellular double-stranded DNA not only in response to viral infection but also under autoimmune conditions.. Measuring the levels of cyclic GMP-AMP (cGAMP) as a second messenger of cGAS activation is important to elucidate the physiological and pathological roles of cGAS.



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    (A) Experimental design of the anti-IL-1β antibody study in the FOP mouse model. Mice received weekly intraperitoneal injections beginning at P27 (day 0) of either vehicle (saline), <t>IgG2A</t> isotype control antibody, or the anti-IL-1β monoclonal antibody 01BSUR (10 mg/kg). Trauma-induced HO was generated using a ball-drop injury model applied to the right hind limb at P28. HO formation was quantified by µCT at day 21. Created with Biorender.com. (B) Quantification of trauma-induced HO volume measured by µCT. Each point represents an individual mouse, and boxes show group distributions. Treatment with the anti-IL-1β antibody 01BSUR significantly reduced HO volume compared with both vehicle-treated mice (p = 0.0087) and IgG2A isotype controls (p = 0.0095). (C) Representative three-dimensional µCT reconstructions of skeletal structures from each treatment group. Areas of HO formation are highlighted. Extensive HO is observed in vehicle- and IgG2A-treated mice, whereas mice treated with the anti-IL-1β antibody show markedly reduced HO at the injury site. Representative images were chosen based on the mean HO volume of each group.
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    Image Search Results


    (A) Experimental design of the anti-IL-1β antibody study in the FOP mouse model. Mice received weekly intraperitoneal injections beginning at P27 (day 0) of either vehicle (saline), IgG2A isotype control antibody, or the anti-IL-1β monoclonal antibody 01BSUR (10 mg/kg). Trauma-induced HO was generated using a ball-drop injury model applied to the right hind limb at P28. HO formation was quantified by µCT at day 21. Created with Biorender.com. (B) Quantification of trauma-induced HO volume measured by µCT. Each point represents an individual mouse, and boxes show group distributions. Treatment with the anti-IL-1β antibody 01BSUR significantly reduced HO volume compared with both vehicle-treated mice (p = 0.0087) and IgG2A isotype controls (p = 0.0095). (C) Representative three-dimensional µCT reconstructions of skeletal structures from each treatment group. Areas of HO formation are highlighted. Extensive HO is observed in vehicle- and IgG2A-treated mice, whereas mice treated with the anti-IL-1β antibody show markedly reduced HO at the injury site. Representative images were chosen based on the mean HO volume of each group.

    Journal: bioRxiv

    Article Title: Gut microbiome-dependent IL-1 signaling is a mediator of ACVR1 R206H -driven heterotopic ossification

    doi: 10.64898/2026.04.05.716562

    Figure Lengend Snippet: (A) Experimental design of the anti-IL-1β antibody study in the FOP mouse model. Mice received weekly intraperitoneal injections beginning at P27 (day 0) of either vehicle (saline), IgG2A isotype control antibody, or the anti-IL-1β monoclonal antibody 01BSUR (10 mg/kg). Trauma-induced HO was generated using a ball-drop injury model applied to the right hind limb at P28. HO formation was quantified by µCT at day 21. Created with Biorender.com. (B) Quantification of trauma-induced HO volume measured by µCT. Each point represents an individual mouse, and boxes show group distributions. Treatment with the anti-IL-1β antibody 01BSUR significantly reduced HO volume compared with both vehicle-treated mice (p = 0.0087) and IgG2A isotype controls (p = 0.0095). (C) Representative three-dimensional µCT reconstructions of skeletal structures from each treatment group. Areas of HO formation are highlighted. Extensive HO is observed in vehicle- and IgG2A-treated mice, whereas mice treated with the anti-IL-1β antibody show markedly reduced HO at the injury site. Representative images were chosen based on the mean HO volume of each group.

    Article Snippet: IgG2A isotype antibody from R&D Systems (catalog no. MAB0031) was used as a control.

    Techniques: Saline, Control, Generated